PRODUCTS

ContentUS

Shandong Youyunpu photoelectric Technology Co., LTD

Youyunpu Optoelectronic Tech

Name：Jenny Li

Tel：+86 19963093538

Email：jennyli0927@163.com

Address:Oulong Science and TechnologyPark,Jinma Road,Weifang City,Shandong Province,China

Current location：HOME>PRODUCT>PCR Thermal Cycler>Real-Time PCR System

Real-Time PCR SystemYP-P96

【Introduction】:Youyunpu Optoelectronic Tech specializes in R&D & production of Real-Time PCR System, with custom services. Complete Real-Time PCR System models, high detection accuracy, trusted by customers.

+86 19963093538

Product Details

I. Product Overview

The YP-P96 Real-Time Quantitative PCR System is mainly used to perform real-time fluorescent quantitative PCR experiments. Through fluorescence excitation and collection, it monitors the experimental process in real time, plots the fluorescence curve from the process data, displays it on the instrument interface in real time, fits and analyzes the experimental data, and finally generates an experimental report in PDF format.

It performs rapid and accurate qualitative and quantitative analysis of target nucleic acids in biological samples taken from other animals (such as blood, body fluids, etc.), and can also analyze special samples for standard curves, melting curves, genotyping, etc.

II. Basic Principle

Principle of the YP-P96 Real-Time Quantitative PCR System: Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA amplification in vitro. A temperature cycle consists of several steps: high-temperature denaturation, low-temperature annealing, and appropriate-temperature extension, enabling rapid amplification of the target gene. It features strong specificity, high sensitivity, simple operation and time saving, representing a major innovation in gene amplification technology. PCR technology can specifically amplify trace amounts of target DNA by millions of times, greatly improving the ability to analyze and detect DNA molecules.

Real-time fluorescent quantitative PCR technology adds corresponding fluorescent dyes or fluorescent-labeled probes on the basis of conventional PCR. During the PCR reaction, the entire PCR process is detected in real time through changes in fluorescent signals. It is a method of monitoring the total product after each PCR cycle with fluorescent chemicals, and quantitatively analyzing specific DNA sequences in the test sample by internal or external reference methods.

III. Product Features

Adopts an advanced thermoelectric cooling system for higher heating and cooling efficiency.
Unique constant current power supply and 6-zone independent temperature control fully ensure stable system performance.
Stable light source and optical path design make fluorescence more authentic and reliable.
Independent fluorescence channels reduce crosstalk between different channels.
Comprehensive analysis functions with customizable report formats to meet different customer scenarios.
Simple and intuitive operating software, easy to learn and use.

IV. Equipment Parameters

Item	Specification
Model	YP-P96
Sample Capacity	96 wells × 0.2 ml
Sample Volume	20–120 μl
Compatible Consumables	0.2 ml single tubes, 8×0.2 ml strip tubes, 96-well plates
Temperature Control Range	0–99 °C
Gradient Temperature Control	6-module gradient, maximum temperature difference 25 °C
Heating Rate	Max. 7 °C/s, average 3.8 °C/s
Cooling Rate	Max. 4.5 °C/s, average 3.5 °C/s
Temperature Accuracy	±0.1 °C
Temperature Uniformity	±0.1 °C
Detection Sensitivity	Single-copy gene detectable
Dynamic Range	10¹–10¹⁰
Repeatability	CV < 3%
Correlation Linearity	0.99–1
Amplification Efficiency	99%–101%
Analysis Algorithms	Amplification curve, standard curve quantification, melting curve, genotyping, SNP (optional)
TouchDown Function	Supports time and temperature increment/decrement modes
Detection Reagents	Custom temperature programs, open real-time PCR reagents
Light Source	4 long-life LED lamps with different filters, specifically and efficiently excite fluorescent molecules of different bands
Transmission Medium	High-temperature-resistant professional optical fiber
Compatible Dyes/Probes	FAM, SYBR Green; HEX, JOE, VIC, TET; ROX, Texas Red; Cy5
Dimensions & Weight	410 × 320 × 196 mm, 11 kg
Power Supply	220 V
Operating System	Standard external Windows computer (Win7 or above); optional Windows all-in-one tablet